Most isolates were unique strains, and the included HAP cases may therefore be characterized as mostly endogenous. Over the study period, no significant clonal spread was noted. Similarity or identity was assessed in 231 bacterial isolates most frequently obtained from HAP patients. A total of 531 bacterial isolates were obtained, of which 267 were classified as etiological agents causing HAP. Included in this study were 330 patients hospitalized between and Decemat departments of anesthesiology and intensive care medicine of four big hospitals in the Czech Republic. Bacterial isolates were compared using pulsed-field gel electrophoresis. This multicenter study of Czech patients with HAP aimed at assessing the clonality of bacterial pathogens causing the condition. Hospital-acquired pneumonia (HAP) is one of the most serious complications in patients staying in intensive care units. Pudová, V Htoutou Sedláková, M Kolář, M PMID:18303199Ĭlonality of Bacterial Pathogens Causing Hospital-Acquired Pneumonia. pneumoniae isolates from other Korean hospitals. The outbreak isolates showed different molecular characteristics from the other K. The MLST method we developed in this study could provide unambiguous and well-resolved data for the epidemiologic study of K. We also found a different distribution of CCs between ESBL-producing and -nonproducing K. pneumoniae isolates from other Korean hospitals (ST20 and SHV-12). Most of the outbreak isolates belonged to a certain clone (ST2), and they produced SHV-1 and CTX-M14 enzymes, which was a different feature from that of the K. The result of MLST analysis was concordant with that of pulsedfield gel electrophoresis. Overall, a total of 37 sequence types (STs) and six clonal complexes (CCs) were identified among the 82 K. pneumoniae based on five housekeeping genes was developed and was evaluated for 43 ESBL-producing isolates from an outbreak as well as 38 surveillance isolates from Korea and also a reference strain. A new multilocus sequence typing (MLST) scheme for K. In this study, we investigated the molecular characteristics of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolates that were recovered from an outbreak in a Korean hospital. Ko, Kwan Soo Yeom, Joon-Sup Lee, Mi Young Peck, Kyong Ran PMID:25341126Ĭlonal Dissemination of Extended-Spectrum β-Lactamase (ESBL)-Producing Klebsiella pneumoniae Isolates in a Korean Hospital pneumoniae populations were largely nonoverlapping, isolates with combined virulence and resistance features were detected. In addition, we created a freely accessible database, BIGSdb-Kp, to enable rapid extraction of medically and epidemiologically relevant information from genomic sequences of K. A total of 694 highly conserved genes were included in a core-genome multilocus sequence typing scheme, and cluster analysis of the data enabled precise definition of globally distributed hypervirulent and multidrug-resistant CGs. pneumoniae isolates, mostly of serotypes K1 and K2, and compared the genomes with 119 publicly available genomes. pneumoniae CGs on the basis of genome-wide sequence variation and to provide a simple bioinformatics tool to extract virulence and resistance gene data from genomic data. Multidrug-resistant and highly virulent Klebsiella pneumoniae isolates are emerging, but the clonal groups (CGs) corresponding to these high-risk strains have remained imprecisely defined.
Genomic Definition of Hypervirulent and Multidrug-Resistant Klebsiella pneumoniae Clonal Groupsīialek-Davenet, Suzanne Criscuolo, Alexis Ailloud, Florent Passet, Virginie Jones, Louis Delannoy-Vieillard, Anne-Sophie Garin, Benoit Le Hello, Simon Arlet, Guillaume Nicolas-Chanoine, Marie-Hélène Decré, Dominique
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